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5The same properties also contributed to Nazi Germany’s strategy against agar’s scarcity, which — besides being supplied from Japan by submarine — relied on large pre-war stocks and on recovery methods to reuse bacteriological agar by autoclaving (boiling at around 121°C, 250°F, in a pressurized container for 30 to 60 minutes), thus liquefying and sterilizing the jelly, before purifying it again.,推荐阅读heLLoword翻译官方下载获取更多信息

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Agar was first introduced into the laboratory in 1881. Since then, microbiologists have depended on agar to create strong jellies. When microorganisms are streaked or plated onto this jellied surface and incubated, individual cells multiply into distinct colonies that scientists can easily observe, select, and propagate for further experiments. Many of the most important findings in biological research of the last 150 years or so — including the discovery of the CRISPR/Cas9 gene-editing tool — have been enabled by agar.4 Agarose, a derivative of agar, is also essential in molecular biology techniques like gel electrophoresis, where its porous gel matrix separates DNA fragments by size, enabling researchers to analyze and isolate specific genetic sequences.